Series in MICROSYSTEMS
edited by P. A. Besse,
M. Gijs,
R. S. Popovic,
Ph. Renaud
Vol. 18:
Klaus Heinrich Ehrmann
Microfabricated
NMR-Probes for the
Analysis of Living Cells.
2006; VIII, 160 p.; 64,00. ISBN 3-86628-095-5,
This book
describes the development of microfabricated probe heads for Nuclear Magnetic
Resonance (NMR) spectroscopy of living cells. Two types of microprobes that
differ regarding their sample interface have been developed: The first type of
microprobes confines the sample in a microchannel. These probes are inspired by
conventional probe heads and are based on microfabricated solenoids and
Helmholtz coils. The second type of microprobes imitates Petri dishes used in
biological research. The sample is immobilized on the probe surface above a
planar microcoil. The performance in NMR of the different microprobes is
assessed in detail using theoretic models based on finite element simulation
and NMR experiments with water and sucrose samples. Devices for on-chip
placement, concentration and perfusion of living cell are integrated into the
probes and experimentally tested. NMR spectra of living cells are taken
employing both types of microprobes. The book finishes with a comparison of the
two types of microprobes with respect to their performance in NMR and in
manipulating living cells.
Klaus Heinrich Ehrmann was born in Crailsheim, Germany, in
1976. He received his
diploma in Microsystems from the Albert-Ludwigs-Universität Freiburg, Germany,
in 2002 after carrying out his diploma project at Imego AB. Göteborg, Sweden.
He then joined the Institute of Microelectronics and Microsystems at the Ecole
Polytechnique Fédérale de Lausanne (EPFL), Switzerland, as a research and
teaching assistant. In parallel he completed his degree in business management.
In 2006 he obtained his Ph.D. for his work on microfabricated NMR probes for
biological analyses.
Keywords: Nuclear Magnetic Resonance (NMR),
microfabrication, microcoils, microfluidics, cell analysis.
Direkt bestellen bei / to order directly from: Hartung.Gorre@t-online.de