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Series in MICROSYSTEMS

edited by P. A. Besse, J. Brugger, M. Gijs, R. S. Popovic, Ph. Renaud

Vol. 18:

Klaus Heinrich Ehrmann

Microfabricated NMR-Probes for the
Analysis of Living Cells.

2006; VIII, 160 p.; 64,00. ISBN 3-86628-095-5,

This book describes the development of microfabricated probe heads for Nuclear Magnetic Resonance (NMR) spectroscopy of living cells. Two types of microprobes that differ regarding their sample interface have been developed: The first type of microprobes confines the sample in a microchannel. These probes are inspired by conventional probe heads and are based on microfabricated solenoids and Helmholtz coils. The second type of microprobes imitates Petri dishes used in biological research. The sample is immobilized on the probe surface above a planar microcoil. The performance in NMR of the different microprobes is assessed in detail using theoretic models based on finite element simulation and NMR experiments with water and sucrose samples. Devices for on-chip placement, concentration and perfusion of living cell are integrated into the probes and experimentally tested. NMR spectra of living cells are taken employing both types of microprobes. The book finishes with a comparison of the two types of microprobes with respect to their performance in NMR and in manipulating living cells.

Klaus Heinrich Ehrmann was born in Crailsheim, Germany, in 1976. He received his diploma in Microsystems from the Albert-Ludwigs-Universität Freiburg, Germany, in 2002 after carrying out his diploma project at Imego AB. Göteborg, Sweden. He then joined the Institute of Microelectronics and Microsystems at the Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland, as a research and teaching assistant. In parallel he completed his degree in business management. In 2006 he obtained his Ph.D. for his work on microfabricated NMR probes for biological analyses.

Keywords: Nuclear Magnetic Resonance (NMR), microfabrication, microcoils, microfluidics, cell analysis.

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