Series in
MICROSYSTEMS
edited by P. A. Besse,
J. Brugger,
M. Gijs,
R. S. Popovic,
Ph. Renaud
Vol. 19:
Pontus Linderholm
Two-dimensional Microimpedance
Imaging for Cell Culture Monitoring
2006,
XXVIII, 252 p.; € 64,00. ISBN 3-86628-107-2
Three-dimensional
tissue cultures are becoming increasingly prevalent in pharmacological and
biomedical research. Measuring the thickness and permeability of in-vitro
tissue is, however, wrought with difficulties. In this thesis, Linderholm
proposes a low-cost technology, based on planar microelectrodes integrated
directly into culture chambers, which allows creating images of the vertical
cross-sections of growing tissue. It is shown how this imaging technique can be
applied to measuring tissue growth, cell migration and drug-induced
permeability changes in the cell membranes. A substantial part of the work is
also devoted to calculating the electric field using conformal mapping, and
modeling the impedance spectra of four-point measurements.
Pontus Linderholm
received his M.Sc in Engineering Biology from the University of Linköping,
Sweden, in 2001. He joined the Institute of Microsystems of the EPFL in 2002,
where he developed a miniaturized impedance imaging sensor as part of his PhD
work. Linderholm has coauthored 10 scientific publications, and currently lives
in Stockholm, Sweden.
Keywords: miniaturized impedance tomography, electrical imaging, cell
culture, four-electrode, conformal mapping, microelectrode, BioMEMS.
Direkt bestellen bei / to order directly from: Hartung.Gorre@t-online.de